A Method Used to Insert or Transform Cells with a Plasmid

question 32

Multiple Choice

A method used to insert or transform cells with a plasmid is to:

• A) add the DNA to bacterial cells that have been lightly treated with lysozyme to produce "holes" in the cell wall.
• B) add the DNA to a heated suspension of cells at 42°C.
• C) treat the bacteria with Ca²⁺, add the DNA, and briefly heat to 42°C.
• D) incubate the DNA with the cells overnight at 4°C.
• E) mixing plasmids with an extract of broken cells.

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