All Are True for CDNA Libraries EXCEPT

question 2

Multiple Choice

All are true for cDNA libraries EXCEPT:

• A) reverse transcriptase synthesizes a DNA strand complement of the mRNA templates.
• B) mRNA templates are isolated using oligo (dA)-cellulose chromatography.
• C) linkers are added and the cDNA is cloned into suitable vectors.
• D) the cDNA are copies from mRNA templates.
• E) all are true.

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Definitions:

Nuclear Division

The process by which a nucleus divides, resulting in the segregation of the genome to distinct nuclei.

Cytoplasmic Division

The process by which a cell divides its cytoplasm to form two daughter cells, typically occurs during cytokinesis.

G₁ Phase

The first phase of the cell cycle in which the cell grows and prepares DNA synthesis.

G₂ Phase

The phase in the cell cycle that follows DNA synthesis (S phase) and precedes mitosis (M phase); it is a period of growth and preparation for cell division.