The Correct Sequence of Procedures in the Southern Blotting (Hybridization)

question 20

Multiple Choice

The correct sequence of procedures in the Southern blotting (hybridization) technique is:
A.hybridization with radioactive probE.
B.agarose gel electrophoresis and visualize bands.
C.transfer (blot) to nitrocellulose filter.
D.digest DNA with restriction nucleases.
E.expose filter to X-ray film, develop and observe.

• A) B, A, C, E, D
• B) D, C, B, A, E
• C) C, D, B, E, A
• D) D, B, C, A, E
• E) A, B, C, D, E

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Definitions:

Ecological Validity

The extent to which research findings accurately represent real-world settings.

Experimental Method

A research method in which variables are manipulated and the effects are observed and measured.

Connectionist Models

Computational models in cognitive science that simulate the neural connections in the brain, emphasizing parallel processing of information.

Central Processor

A term relating to the central executive in Baddeley and Hitch’s model of working memory, involved in reasoning, decision making, and updating and manipulating the contents of working memory.