How Is a Fluorescent Tag That Is Unique for Each

question 30

Multiple Choice

How is a fluorescent tag that is unique for each nucleotide incorporated into the newly synthesized DNA using the chain termination protocol for DNA sequencing?

• A) Each of the ddNTPs is labeled with a specific fluorophore
• B) The primer strand is labeled with a specific fluorophore
• C) The template strand is labeled prior to the experiment with complimentary fluorophores
• D) The 5'-end is labeled with a fluorophore.
• E) All dNTPs are labeled with a fluorophore

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Definitions:

Cerebrum

The largest part of the brain, responsible for voluntary muscular activity, vision, speech, taste, hearing, thought, and memory.

Limbic System

A complex system of nerves and networks in the brain, involved in basic emotions and drives.

Hypothalamus

A region of the brain responsible for hormone production and regulation of vital bodily functions such as temperature and hunger.

Pons

A part of the brain located in the brainstem, vital for regulating breathing, communication between different parts of the brain, and relaying sensory information.