You Are a Scientist Who Wants to Produce Large Amounts

question 3

Essay

You are a scientist who wants to produce large amounts of the protein insulin that is used to facilitate the entry of glucose into cells. You know that insulin genes may be introduced into bacteria by way of a plasmid vector. These bacteria may then be used as 'micro-manufacturers' of insulin. Your laboratory technicians explore various methods of producing the desired bacteria, but you are not sure how many and which of the bacterial cells have been transfected (have the plasmid with the insulin gene). Propose a way to determine which bacterial cells have been transfected with the engineered plasmid and are able to produce insulin.

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Definitions:

P-Value

The p-value is a measure in statistics that helps determine the significance of results from a hypothesis test, indicating the probability of observing the results given that the null hypothesis is true.

Level Of Significance

A threshold or criterion for judging whether a statistical result is significant, commonly set at values like 0.05 or 0.01, indicating the risk of a Type I error.

Null Hypothesis

A default hypothesis that there is no significant difference or effect, tested against to determine the likelihood of the alternative hypothesis.

One-Tail Test

A statistical test in which the region of rejection is on only one side of the sampling distribution, used to determine if a parameter is greater than or less than a certain value.