Why Do Phenol and Buffered Saline Separate into Two Phases

question 137

Multiple Choice

Why do phenol and buffered saline separate into two phases when one centrifuges the mixture during the DNA extraction procedure?

• A)They are soluble.
• B)They are miscible.
• C)They are immiscible.
• D)One freezes and the other does not.
• E)They are both hydrophobic.

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Definitions:

Relief Valve

A safety valve designed to automatically release pressure from a vessel or system when the pressure exceeds safe levels, preventing potential damage or explosion.

Flow Control Valve

Used in hydraulic circuits to regulate the speed of actuators. Functions by defining a flow area and is rated by operating pressure and capacity. It can be used in meter-in or meter-out applications. Two general types are used: bleed-off and fixed orifice.

Pitman Shaft

Part of the steering mechanism in vehicles, connecting the steering box to the steering linkage, enabling the transfer of motion to steer the wheels.

Splines

Radial lands that either protrude from or are milled into a shaft to permit it to engage with female splines for the purpose of transmitting torque.