In the PCR Quantification Procedure Involving Molecular Beacons, What Causes

question 51

Multiple Choice

In the PCR quantification procedure involving molecular beacons, what causes the fluorochrome to fluoresce?

• A)The degradation of the reporter oligonucleotide by DNA polymerase endonuclease activity degrades the fluorochrome.
• B)The degradation of the reporter oligonucleotide by DNA polymerase exonuclease activity separates the fluorochrome from the quencher.
• C)The degradation of the reporter oligonucleotide by DNA polymerase exonuclease activity degrades the fluorochrome.
• D)The degradation of the reporter oligonucleotide by DNA polymerase endonuclease activity separates the fluorochrome from the quencher.
• E)DNA polymerase combines the fluorochrome and the quencher.

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Definitions:

Sleep Deprived

Sleep deprived describes a state resulting from not getting enough rest, leading to physical and cognitive impairments.

Cortisol

A steroid hormone produced by the adrenal gland, released in response to stress and low blood-glucose concentration.

Metabolic Rate

The rate at which the body uses energy, measured in calories, to maintain vital functions while at rest.

Stress Hormone

Hormones, such as cortisol, released in the body's response to stress, affecting various bodily functions.