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What Amino Acid Is Made to Replace Another Amino Acid α\alpha

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What amino acid is made to replace another amino acid in membrane-spanning α\alpha -helices by site-directed mutagenesis of the gene coding for the protein? This is done in order to determine how close together two such α\alpha -helices are in the structure of an integral protein.The amino acid is added to facilitate the formation of crosslinks between α\alpha -helices that are in close proximity.


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