In the PCR Quantification Procedure Involving Molecular Beacons, What Causes

question 51

Multiple Choice

In the PCR quantification procedure involving molecular beacons, what causes the fluorochrome to fluoresce?

• A)The degradation of the reporter oligonucleotide by DNA polymerase endonuclease activity degrades the fluorochrome.
• B)The degradation of the reporter oligonucleotide by DNA polymerase exonuclease activity separates the fluorochrome from the quencher.
• C)The degradation of the reporter oligonucleotide by DNA polymerase exonuclease activity degrades the fluorochrome.
• D)The degradation of the reporter oligonucleotide by DNA polymerase endonuclease activity separates the fluorochrome from the quencher.
• E)DNA polymerase combines the fluorochrome and the quencher.

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Definitions:

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