A Preparation of Submitochondrial Particles Is Made

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The Answer is Urea treatment removes the F1 spheres from ATP synthase.As a result,the membrane is no longer able to maintain the proton gradient.Protons leak out of the F0 base piece in an uncontrolled fashion when the headpiece is removed and ATP cannot be made because the headpiece that makes it is gone.DNP also uncouples ATP production from electron transport.It causes the membrane to become permeable to protons and thus dissipates the proton gradient,making ATP production by ATP synthase impossible.DNP combines with protons and,because of its lipid solubility,carries the protons across the inner mitochondrial membrane down their electrochemical gradient.

A Preparation of Submitochondrial Particles Is Made

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