You Measure the Initial Velocity of an Enzyme in the Absence

question 31

Essay

You measure the initial velocity of an enzyme in the absence and presence of an inhibitor. In each case the inhibitor is at 10 µM. Show the primary data for all three cases and the Lineweaver–Burk plot. Calculate the KM and Vmax for each case both graphically and mathematically. Determine the mechanism for each inhibitor and where they will interact on the enzyme.
$\begin{array}{c}\underline{\text { Initial Velocity ( } \mu \mathrm { mole } / \mathrm { ml } \mathrm { min } )}\\\begin{array} { c c c c } [ \mathrm { S } ] \mathrm { mM } & \begin{array} { c } \text { Enzyme } \\\text { alone }\end{array} & \begin{array} { c } \text { Enzyme } \\+ \text { inhibitor 1 }\end{array} & \begin{array} { c } \text { Enzyme } \\+ \text { inhibitor 2 }\end{array} \\0.33 & 1.65 & 1.05 & 0.79 \\0.50 & 2.13 & 1.43 & 1.02 \\1.00 & 2.99 & 2.22 & 1.43 \\2.00 & 3.72 & 3.08 & 1.79 \\5.00 & 4.00 & 3.80 & 2.00\end{array}\end{array}$

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Definitions:

Unconditioned Stimulus

A stimulus that naturally and automatically triggers a response without needing to be learned.

Comparator Model

A psychological theory suggesting that individuals compare their own outcomes with those of others to assess fairness and satisfaction.

Conditioned Stimulus

A previously neutral signal that, through association with an unconditioned stimulus, comes to elicit a conditioned response as part of classical conditioning.