A Technique Called Flow Cytometry Is Used by Scientists and Researchers

question 29

Multiple Choice

A technique called flow cytometry is used by scientists and researchers to count or sort cells based on specific properties. By labeling cellular DNA with a fluorescent dye, flow cytometry can sort cells based on the amount of DNA present, thereby making it possible to distinguish between cells that are in different stages of the cell cycle. Flow cytometry is especially useful to the medical community because it can help with the diagnosis of certain types of cancers. Below is a typical set of data that you might obtain when running a flow cytometry experiment using a sample of healthy skin cells. Use this figure to help you answer the following questions. After reading the paragraph below, answer the questions that follow.
-Which peak represents cells that contain the most DNA?

• A) A
• B) B
• C) C
• D) It cannot be determined from this figure.

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Definitions:

Kwashiorkor

A condition occurring in children who suffer severe protein and calorie shortages, which is characterized by stunted growth, a protuberant belly, and extreme apathy. Although therapeutic diets can reverse some effects, cognitive impairments are likely to persist.

Protein

A large biomolecule, or macromolecule, consisting of one or more long chains of amino acid residues, essential for the structure, function, and regulation of the body's cells, tissues, and organs.

Calorie Shortages

Instances where there is a deficit in the number of calories consumed versus those required for maintaining healthy bodily functions.

Fetal Alcohol Syndrome

A condition in a child resulting from alcohol exposure during the mother's pregnancy, causing growth problems, facial anomalies, and developmental disabilities.