A Technique Called Flow Cytometry Is Used by Scientists and Researchers

question 65

Multiple Choice

A technique called flow cytometry is used by scientists and researchers to count or sort cells based on specific properties. By labeling cellular DNA with a fluorescent dye, flow cytometry can sort cells based on the amount of DNA present, thereby making it possible to distinguish between cells that are in different stages of the cell cycle. Flow cytometry is especially useful to the medical community because it can help with the diagnosis of certain types of cancers. Below is a typical set of data that you might obtain when running a flow cytometry experiment using a sample of healthy skin cells. Use this figure to help you answer the following questions. After reading the paragraph below, answer the questions that follow.
-Which peak corresponds to cells in G1? In S phase? In G2?

• A) A: G1; B: G2; C: S
• B) A: G2; B: S; C: G1
• C) A: S; B: G1; C: G2
• D) A: G1; B: S; C: G2

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Definitions:

Empirical Literature

Published research or studies that are based on observed and measured phenomena, derived from practical experience rather than theory or pure logic.

Analogue Measures

Tools or methods that simulate real-world processes or environments for the purpose of measurement or experimentation.

Generalise

The process of applying the results or conclusions from a specific instance to a broader situation or population.

Nature-Nurture Controversy

A debate concerning the relative importance of an individual's innate qualities (nature) versus personal experiences (nurture) in determining or causing physical and behavioral traits.