You Are Setting Up a PCR Reaction and Add a Primer

question 66

Multiple Choice

You are setting up a PCR reaction and add a primer for one end of the target sequence, but you forget to add the primer for the other end of the target sequence. If you added the other necessary components, what do you expect to observe after running the PCR reaction for 40 cycles?

• A) The PCR reaction will produce billions of copies of the target DNA.
• B) The PCR reaction will produce millions of copies of the target DNA.
• C) The PCR reaction will produce one copy of the target DNA.
• D) The PCR reaction will produce zero copies of the target DNA.

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Definitions:

Cyclopentanone

A cyclic ketone with a five-membered ring, known for its distinctive odor and use in organic synthesis.

1,2-Epoxy-1-Methylcyclopentane

1,2-Epoxy-1-Methylcyclopentane is an epoxy compound featuring a cyclopentane ring with a methyl group substitution and an epoxide functional group.

Trans-1,2-Cyclopentanediol

A compound featuring a cyclopentane ring with hydroxyl groups at the 1 and 2 positions on opposite sides, denoting its trans configuration.

Cyclopentene

A cyclic alkene with a five-membered ring, used in the synthesis of more complex organic compounds.