Some Restriction Enzymes Do Not Leave Sticky Ends When Cutting

question 70

Multiple Choice

Some restriction enzymes do not leave sticky ends when cutting DNA molecules; rather, they cut a restriction site down the middle and leave "blunt-ended" DNA molecules, which do not have any single-stranded nucleotide extensions. Which of the following is not a likely outcome when trying to insert a gene into a plasmid when both have been cut with the same "blunt-ended" restriction enzyme?

• A) The gene might be inserted into the plasmid by forming hydrogen bonds.
• B) The gene might be inserted into the plasmid multiple times in a row.
• C) The gene might insert into the plasmid in the proper (forward) orientation.
• D) The gene might insert into the plasmid in the wrong (backward) orientation.

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