Suppose a Researcher Is Interested in Using the CRISPR-Cas9 System

question 32

Multiple Choice

Suppose a researcher is interested in using the CRISPR-Cas9 system to knock out the phosphofructokinase gene of a rat skin cell that codes for an enzyme that is critical for glycolysis to occur. The researcher added the Cas9 enzyme to the cell but forgot to add the guide RNA. What result do you think the researcher will observe?

• A) Glycolysis will proceed normally in the cell.
• B) The phosphofructokinase enzyme will be expressed but will have lower activity.
• C) Cas9 will specifically cut the phosphofructokinase gene.
• D) There will be mutations in the phosphofructokinase gene.

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