Some Restriction Enzymes Do Not Leave Sticky Ends When Cutting

question 70

Multiple Choice

Some restriction enzymes do not leave sticky ends when cutting DNA molecules; rather, they cut a restriction site down the middle and leave "blunt-ended" DNA molecules, which do not have any single-stranded nucleotide extensions. Which of the following is not a likely outcome when trying to insert a gene into a plasmid when both have been cut with the same "blunt-ended" restriction enzyme?

• A) The gene might be inserted into the plasmid by forming hydrogen bonds.
• B) The gene might be inserted into the plasmid multiple times in a row.
• C) The gene might insert into the plasmid in the proper (forward) orientation.
• D) The gene might insert into the plasmid in the wrong (backward) orientation.

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Definitions:

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The portion of net income distributed to shareholders in the form of dividends, expressed as a percentage of the company's total net income.

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A financial measure that indicates the theoretical value per share of a company if it were to liquidate, calculated by dividing total equity by the number of outstanding shares.

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A valuation metric for companies, calculated by dividing the current market price of a stock by its earnings per share (EPS).

Dividend Yield Ratio

A financial ratio that indicates how much a company pays out in dividends each year relative to its stock price, typically expressed as a percentage.