Suppose a Researcher Is Interested in Using the CRISPR-Cas9 System

question 32

Multiple Choice

Suppose a researcher is interested in using the CRISPR-Cas9 system to knock out the phosphofructokinase gene of a rat skin cell that codes for an enzyme that is critical for glycolysis to occur. The researcher added the Cas9 enzyme to the cell but forgot to add the guide RNA. What result do you think the researcher will observe?

• A) Glycolysis will proceed normally in the cell.
• B) The phosphofructokinase enzyme will be expressed but will have lower activity.
• C) Cas9 will specifically cut the phosphofructokinase gene.
• D) There will be mutations in the phosphofructokinase gene.

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Definitions:

Financing Costs

Expenses incurred by a company in the process of raising funds, including interest payments, issuance costs of securities, and other related expenses.

Incremental Cash Flows

The additional cash flows from undertaking a project or investment.

NWC

Net Working Capital, which is calculated as current assets minus current liabilities, indicating the company's short-term financial health.

Sunk Cost

A cost that has already been incurred and cannot be recovered, which should not influence future decisions.