Gene Knockdown Is a Procedure Used to Reduce Gene Expression

question 11

Multiple Choice

Gene knockdown is a procedure used to reduce gene expression in cells or organisms so that scientists can investigate gene function. While there are several ways to knock down gene expression, RNAi is one of the most commonly used approaches. Suppose that a research team wants to investigate where a new gene X is expressed in the fruit fly, Drosophila melanogaster. Using RNAi, they obtained the results shown below examining the expression of gene X in four types of tissues.
-Which of the following components could the researchers have used to successfully perform RNAi against gene X?

• A) an siRNA molecule that has a complementary sequence to that of gene X
• B) a transcription factor that binds to and activates the gene X promoter
• C) an enzyme that helps fold the X protein after it is translated

---

Definitions:

Sleep Deprivation

The condition of not having enough sleep, which can impair cognitive function, mood, and physical health.

Serotonin

A chemical messenger vital for regulating mood, digestion, sleep, and various bodily activities.

Suprachiasmatic Nucleus

A tiny region in the brain's hypothalamus responsible for controlling circadian rhythms, functioning as the body's internal clock.

Sleep Cycles

The progression through various stages of sleep, including REM and non-REM phases, which repeat approximately every 90 minutes.