Which Procedure Is Not Required When the Shotgun Approach to Sequencing

question 13

Multiple Choice

Which procedure is not required when the shotgun approach to sequencing is modified as sequencing by synthesis, in which many small fragments are sequenced simultaneously?

• A) use of restriction enzymes
• B) sequencing each fragment
• C) cloning each fragment into a plasmid
• D) ordering the sequences
• E) PCR amplification

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Definitions:

Internal Organs

Vital parts within the body, such as the heart, lungs, and liver, that perform essential functions required for survival.

Serviceable Associated Habits

Habits that are beneficial or useful in performing daily activities or achieving long-term goals.

Darwin

Refers to Charles Darwin, a naturalist known for his theory of evolution through natural selection, which had a profound impact on the understanding of species development.

Learned Behaviors

Actions or reactions developed as a result of experience or conditioning rather than innate.