What Is the Most Logical Sequence of Steps for Splicing

question 8

Multiple Choice

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I.Transform bacteria with a recombinant DNA molecule.
II.Cut the plasmid DNA using restriction enzymes.
III.Extract plasmid DNA from bacterial cells.
IV.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V.Use ligase to seal plasmid DNA to nonplasmid DNA.

• A) I, II, IV, III, V
• B) II, III, V, IV, I
• C) III, II, IV, V, I
• D) III, IV, V, I, II
• E) IV, V, I, II, III