Please Use the Following Information to Answer the Question(s) Below

question 51

Multiple Choice

Please use the following information to answer the question(s) below.
A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to the antibiotics ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
-Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow

• A) in the nutrient broth only.
• B) in the nutrient broth and the tetracycline broth only.
• C) in the nutrient broth, the ampicillin broth, and the tetracycline broth.
• D) in all four types of broth.
• E) in the ampicillin broth and the nutrient broth.

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Definitions:

General Adaptation Syndrome

A theoretical model describing the body's short-term and long-term reactions to stress, involving stages of alarm, resistance, and exhaustion.

Tend-and-befriend Response

A behavioral response to stress that involves seeking social support and nurturing others, theorized to be more common in females.

Problem-focused Coping

A strategy aimed at directly confronting or managing a problem to alleviate stress or conflict.

Stress

The body's response to any demand or challenge, which can be triggered by both positive and negative experiences and can affect mental and physical health.