In Experimental Tests of Enzyme Evolution, a Gene Encoding an Enzyme

question 26

Multiple Choice

In experimental tests of enzyme evolution, a gene encoding an enzyme was subjected to multiple cycles of random mutagenesis and selection for altered substrate specificity. The resulting enzyme had altered substrate specificity and multiple amino acid changes. Where in the enzyme would you expect these amino acid changes to be located?

• A) only in or near the active site
• B) only in the hydrophobic interior of the folded protein
• C) only at surface sites distant from the active site
• D) in or near the active site and at surface sites distant from the active site

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Definitions:

Palpate

The act of examining or exploring by touch, used in clinical assessments to detect abnormalities or evaluate the condition of muscles, organs, and other body parts.

Inspect

In a medical context, to "inspect" means to visually examine the body or body part for signs of disease, injury, or abnormality.

General Survey

An initial part of a physical examination where a healthcare provider observes a patient’s overall appearance and behavior, gathering significant information at first glance.

Skin Turgor

The elasticity of the skin, often assessed through a pinch test to evaluate hydration status and the overall health of the connective tissue.