In Experimental Tests of Enzyme Evolution, a Gene Encoding an Enzyme

question 26

Multiple Choice

In experimental tests of enzyme evolution, a gene encoding an enzyme was subjected to multiple cycles of random mutagenesis and selection for altered substrate specificity. The resulting enzyme had altered substrate specificity and multiple amino acid changes. Where in the enzyme would you expect these amino acid changes to be located?

• A) only in or near the active site
• B) only in the hydrophobic interior of the folded protein
• C) only at surface sites distant from the active site
• D) in or near the active site and at surface sites distant from the active site

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Definitions:

Credit Totals

The aggregate amount of credit entries recorded in a financial system or ledger.

Trial Balance

A spreadsheet where the totals of all ledger balances are gathered into equal totals in debit and credit columns for accounts.

Debit Column

A column in financial accounting used to record entries that decrease liabilities and increase assets or expenses.

Credit Column

A section on the right side of an accounting ledger used to record increases in liability, equity accounts, and revenue, or decreases in assets and expense accounts.