Please Use the Following Information to Answer the Question(s) Below

question 51

Multiple Choice

Please use the following information to answer the question(s) below.
A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to the antibiotics ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
-Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow

• A) in the nutrient broth only.
• B) in the nutrient broth and the tetracycline broth only.
• C) in the nutrient broth, the ampicillin broth, and the tetracycline broth.
• D) in all four types of broth.
• E) in the ampicillin broth and the nutrient broth.

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Definitions:

Care Coordination

The deliberate organization of patient care activities and sharing information among all participants concerned with a patient's care to achieve safer and more effective care.

Cost Effective

An economic analysis that compares the relative expenses and outcomes of different courses of action to determine the most efficient way to achieve a specific goal.

Chronically Ill

Referring to individuals suffering from one or more long-term health conditions which may not be curable and require ongoing management.

Community Health Nurse

A nursing professional who works in public health settings to promote and protect the health of populations in the community, focusing on prevention and education.