Please Use the Following Information to Answer the Question(s) Below

question 51

Multiple Choice

Please use the following information to answer the question(s) below.
A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to the antibiotics ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
-Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow

• A) in the nutrient broth only.
• B) in the nutrient broth and the tetracycline broth only.
• C) in the nutrient broth, the ampicillin broth, and the tetracycline broth.
• D) in all four types of broth.
• E) in the ampicillin broth and the nutrient broth.

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Definitions:

Investment Tax Credit

A tax credit offered to businesses to encourage specific types of investments.

Real GDP

The measure of a country's economic output adjusted for price changes, such as inflation or deflation, to reflect the true value of goods and services produced.

Investment

The action or process of allocating resources, usually money, with the expectation of generating an income or profit.

Real GDP

The measure of the value of economic output adjusted for price changes (inflation or deflation), reflecting the real quantity of goods and services produced.