Suzanne Simard and Colleagues Knew That the Same Mycorrhizal Fungal

question 27

Multiple Choice

Suzanne Simard and colleagues knew that the same mycorrhizal fungal species could colonise multiple types of trees. They wondered if the same fungal individual would colonise different trees, forming an underground network that potentially could transport carbon and nutrients from one tree to another (S. Simard et al. 1997. Net transfer of carbon between mycorrhizal tree species in the field. Nature 388:579-82) .
Pots containing seedlings of three different tree species were set up and grown under natural conditions for three years (Fig.A) . Two of the three species (Douglas fir, birch) could form ectomycorrhizal connections with the same fungal species, but the third species (cedar) could not form an ectomycorrhizal connection with the fungal species. In some of the pots, the researchers placed airtight bags over the Douglas fir and birch seedlings and injected carbon dioxide made from carbon-13 into the bags with the Douglas fir and carbon dioxide made from carbon-14 into the bags with the birch. (13C and 14C are different isotopes of carbon that can be detected and measured by researchers.) As the seedlings photosynthesised, the carbon dioxide was converted into sugars that could be tracked and measured by the researchers. The researchers measured whether the sugars in each plant contained only the carbon isotope that was in the air of their plastic bag or also the carbon isotope from the air around the other plant.

Figure A
-Referring to Simard et al. (1997) , what is the result that would most strongly refute their hypothesis? [Hypothesis: Sugars made by one plant during photosynthesis can travel through a mycorrhizal fungus and be incorporated into the tissues of another plant.]

• A) No movement: Carbon-14 is found in the birch seedling's tissues and carbon-13 in the Douglas fir.
• B) Reciprocal exchange: Carbon-14 is found in the Douglas fir seedling's tissues and carbon-13 in the birch.
• C) Either carbon-13 or carbon-14 is found in the fungal tissues.
• D) Either carbon-13 or carbon-14 is found in the cedar seedling's tissues.

---

Definitions:

Hypothetical Incident

An imagined or theoretical situation used for the purpose of analysis, discussion, or problem-solving.

Situational Interview

A job interview method where candidates are asked to describe how they would handle specific job-related situations, testing their problem-solving and decision-making abilities.

Poor Prediction Rate

The occurrence of low accuracy in forecasting future events, outcomes, or behaviors, often due to inadequate information or model flaws.

Ability Test

An assessment used to measure specific skills or competencies, often related to intellectual or physical capabilities.