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Use the following description to answer the question.
On the Bahamian island of Andros, mosquitofish populations live in various, now-isolated freshwater ponds that were once united. Currently, some predator-rich ponds have mosquitofish that can swim in short, fast bursts; other predator-poor ponds have mosquitofish that can swim continuously for a long time. When placed together in the same body of water, the two kinds of female mosquitofish exhibit exclusive breeding preferences.
Which two of the following have operated to increase divergence between mosquitofish populations on Andros?
1) improved gene flow
2) bottleneck effect
3) sexual selection
4) founder effect
5) natural selection


Definitions:

Cardiac Output

The volume of blood the heart pumps through the circulatory system in one minute, an essential parameter in assessing cardiac health.

Diastolic Filling

The phase in the cardiac cycle where the heart chambers fill with blood following relaxation.

Hemoglobin Level

A measurement of the concentration of hemoglobin in a person's blood, indicating the blood's capacity to carry oxygen.

Myocardial Infarction

Necrosis of a portion of cardiac muscle caused by obstruction in a coronary artery.

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