Nucleotides Can Be Radiolabelled Before They Are Incorporated into Newly

question 49

Multiple Choice

Nucleotides can be radiolabelled before they are incorporated into newly synthesised DNA and, therefore, can be assayed to track their incorporation. In a set of experiments, a student-faculty research team used labelled T nucleotides and introduced these into a culture of dividing human cells at specific times. Which of the following questions might be answered by using the method described?

• A) How many cells are produced by the culture per hour?
• B) What is the length of the S phase of the cell cycle?
• C) How many picograms of DNA are made per cell cycle?
• D) When do spindle fibres attach to chromosomes?

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