The Steps Involved in a Single PCR Cycle, in the Correct

question 4

Multiple Choice

The steps involved in a single PCR cycle, in the correct order, are

• A) cool to allow DNA strands to anneal, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
• B) heat to denature DNA strands, cool to allow primer to anneal and to allow DNA polymerase to copy DNA.
• C) heat to denature DNA strands and for primer to anneal, cool to allow DNA polymerase to copy DNA.
• D) cool to denature DNA strands, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
• E) cool to allow primer to anneal, heat to denature DNA strands, cool to allow DNA polymerase to copy DNA.
• A typical PCR cycle has two steps: denaturation, and annealing/extension. The reaction is heated to near boiling to separate the two DNA strands, and then the reaction is cooled to allow the primers to anneal to the DNA and to allow the DNA polymerase to copy the DNA.

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