In Shotgun Sequencing, How Is a Complete Sequence of a Genome

question 8

Multiple Choice

In shotgun sequencing, how is a complete sequence of a genome reconstructed?

• A) The entire genome is directly sequenced chromosome by chromosome.
• B) The genome is fragmented and sequences of each fragment are arranged so that overlapping regions are identified.
• C) The sequences of a large number of individuals is obtained and "averaged" to obtain the complete sequence.
• D) The position of each marker and gene are first mapped relative to regions for which the genomic sequence is not known.

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Definitions:

Pent-1-yne

An alkyne with the chemical formula C5H8, characterized by a triple bond between the first and second carbon atoms in the five-carbon chain.

Transformation

A thorough or dramatic change in form or appearance, often used in the context of chemical reactions.

Reagents

Chemical substances used in reactions to cause a transformation or detect the presence of another substance.

Transformation

A process by which the genetic material of an organism is altered by the integration of new DNA, especially relevant in genetics, molecular biology, and biotechnology.