Gene Methylation Can Be Detected Through the Use of Restriction

question 33

Multiple Choice

Gene methylation can be detected through the use of restriction endonucleases. Usually these are used in pairs where one enzyme will digest only unmethylated DNA in its recognition sequence while the other is insensitive to methylation. Which of the following statements is correct?

• A) The enzyme that is insensitive to methylation serves as a control to make sure the inability of the other enzyme to digest DNA is not due to a mutation.
• B) These are used in pairs since experiments have to be replicated and this one way of performing a replication of the experiment
• C) The experimental design is flawed since there should be a third enzyme that would serve as a positive control
• D) This experimental design is able to detect most of the methylated C residues in a particular region of DNA.

---

Definitions:

Journey Mapping

A visual representation or diagram that outlines the steps customers go through in engaging with a company, with the aim of understanding their experience.

Developing Empathy

Enhancing the ability to understand and share the feelings of another, which can improve interpersonal relations and customer service.

Customer Journey Map

A visual representation of the steps a customer goes through in engaging with a company, from initial awareness to post-purchase experiences.

Customer Experience

The overall perception and interaction a customer has with a business and its products or services throughout the entire customer journey.