You Perform a Cell Free Translation Experiment Like Nirenberg and Matthaei

question 8

Multiple Choice

You perform a cell free translation experiment like Nirenberg and Matthaei, but you forget to write down what nucleotides you added to make the mRNA. You precipitate the translated polypeptides and measure the relative amount of radiolabeled amino acids incorporated into them. You get 25% proline, 25% threonine, 12.5% glutamine, 12.5% lysine, 12.5% asparagine, and 12.5% histidine. What nucleotides and in what % did you add to make the mRNA?

• A) Equal amounts of A, C, U, and G
• B) 50% C and 50% A
• C) 70% C and 30% A
• D) 50% C and 50% U
• E) 50% A and 50% G

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