You Cloned a 2-Kb Fragment of DNA into a Bacterial

question 2

Multiple Choice

You cloned a 2-kb fragment of DNA into a bacterial cloning vector and want to construct a restriction map of the insert.You amplify the 2-kb insert using PCR, purify it, and subject it to differential digestion with the enzymes EcoRI and HindIII, gel-fractionate the digests, and visualize the restriction patterns by staining the gels with ethidium bromide to generate the following results.Using these data, indicate the CORRECT order of restriction sites in the DNA insert.

• A)EcoRI, HindIII, EcoRI, HindIII, EcoRI
• B)HindIII, EcoRI, HindIII, EcoRI, HindIII
• C)HindIII, EcoRI, HindIII, HindIII, EcoRI
• D)HindIII, EcoRI, EcoRI, HindIII, EcoRI
• E)EcoRI, EcoRI, HindIII, EcoRI, HindIII

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Definitions:

Equilibrium Price

A market condition where supply meets demand, and there is no inclination for price to change, leading to market balance.

Mutually Beneficial Trades

Exchanges that occur when all parties involved gain benefits or profits from the transaction.

Total Surplus

The sum of consumer surplus and producer surplus in a market, representing the total net benefit to society from trade.

Consumer Surplus

The separation between what consumers envisage paying for a good or service and the amount they actually disburse.