You Cloned a 2-Kb Fragment of DNA into a Bacterial

question 15

Multiple Choice

You cloned a 2-kb fragment of DNA into a bacterial cloning vector and want to construct a restriction map of the insert.You amplify the 2-kb insert using PCR, purify it, and subject it to differential digestion with the enzymes EcoRI and HindIII, gel-fractionate the digests, and visualize the restriction patterns by staining the gels with ethidium bromide to generate the following results.Using these data, which two loci would be CLOSEST on a genetic map if the frequency of crossing over is equal in this region?

• A)The EcoRI sites that are 100 bp apart would show the smallest distance in cM.
• B)The HindIII sites that are 750 bp apart would show the smallest distance in cM.
• C)The HindIII site and the EcoRI site that are 200 bp apart would show the smallest distances in cM.
• D)The EcoRI site and the HindIII site that are 300 bp apart would show the smallest distance in cM.
• E)The EcoRI sites that are 150 bp apart would show the smallest distance in cM.

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