Which of the Following Is a Disadvantage of Map-Based Sequencing

question 70

Multiple Choice

Which of the following is a disadvantage of map-based sequencing over shotgun sequencing?

• A) Map-based sequencing minimizes the amount of repeat sequencing in which the same region is sequenced several times.
• B) Map-based sequencing yields well-characterized physical maps as well as actual sequences.
• C) The relationship of overlapping fragments to sequences is known before sequencing begins.
• D) Map-based sequencing is more time-consuming because extensive mapping is required before sequencing starts.
• E) Restriction patterns can be used to identify overlapping clones.

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