You Are Attempting to Generate a Physical Map of the Bacteriophage

question 29

Essay

You are attempting to generate a physical map of the bacteriophage $\lambda$ genome using restriction enzyme digestions.You digest the phage DNA with EcoRI or NcoI or both, and observe the fragment sizes listed below.You know that bacteriophage $\lambda$ has a linear, not circular, genome. How many times does the EcoRI enzyme cut the $\lambda$ DNA? How many times does the NcoI enzyme cut the $\lambda$ DNA?

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Definitions:

Cash Over And Short

An account that records the discrepancies between the actual cash received and the expected amount.

Credit Balance

An accounting term that signifies a positive balance in a liability, revenue, or equity account, which increases on the credit side and decreases on the debit side.

Petty Cash Fund

A nominal sum of cash reserved for quick payments of assorted trivial expenses.

Replenishing

Refers to the process of adding stock or supplies to bring quantities back up to predetermined levels.