Dideoxy Nucleotides That Are Used in DNA Sequencing Experiments Terminate

question 30

Multiple Choice

Dideoxy nucleotides that are used in DNA sequencing experiments terminate DNA synthesis because they:

• A)cannot be recognized by DNA polymerases and therefore not incorporated during synthesis.
• B)have methyl groups attached to the 5'position of the sugar and can't be incorporated during synthesis.
• C)are only recognized by reverse transcriptases.
• D)have no 3'-OH group and so no additional nucleotides can be added to them after they are incorporated.
• E)have no phosphate groups.

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