Figure a Below Shows a Restriction Map of a Rare

question 65

Essay

Figure A below shows a restriction map of a rare prokaryotic gene with its direction of transcription indicated by the arrow.Figure B shows the unique restriction sites contained within a plasmid-cloning vector.The blackened region in Figure A represents the amino acid coding sequence of a protein that can be used in humans as a vaccine.The stippled region in Figure B is a highly active, constitutive (unregulated)prokaryotic promoter region.Letters indicate the cleavage sites for different restriction enzymes.Known DNA sequences are indicated by short thick lines.Explain how you would isolate and then insert the coding region (Figure A)under the control of the indicated promoter in the cloning vector (FigureB)to produce large amounts of the protein in bacterial cells.Assume that the cloning vector carries the gene for tetracycline (an antibiotic)resistance.Letters represent different restriction enzymes.

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Definitions:

Beginning Inventory

The value of all products, goods, and materials in stock at the start of an accounting period.

LIFO

"Last In, First Out," an inventory valuation method where the latest items added to the inventory are the first to be sold.

Ending Inventory

The value of goods available for sale at the end of an accounting period, calculated using beginning inventory, purchases, and cost of goods sold.

Units

A measure of quantity, such as items, goods, or services, which can be counted or measured.