In Using a Cloning Vector with a Multiple Cloning Site

question 17

Multiple Choice

In using a cloning vector with a multiple cloning site spliced into the front part of the lacZ gene, production of beta-galactosidase, as shown by the blue color when grown on Xgal, indicates that:

• A)an insert has been placed into the multiple cloning site.
• B)the cells are antibiotic sensitive.
• C)the cells lack plasmids.
• D)the cells have not been successfully transformed.
• E)there is no insert in the multiple cloning site.

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Definitions:

Actor-observer Effect

The tendency to attribute one's own actions to external causes while attributing other people's behaviors to their internal characteristics.

External

Outside of or existing beyond the self or the entity it refers to, often used in contrast with internal.

Internal

Relating to or situated on the inside; intrinsic or inner attributes.

Likert Scale

A psychometric scale commonly involved in research that employs questionnaires. It is used for assessing attitudes by asking respondents to rate their level of agreement with a statement using a choice scale.