A Fragment of DNA Is Cloned into a Plasmid with a Sequencing

question 48

Multiple Choice

A fragment of DNA is cloned into a plasmid with a sequencing primer-binding site.After dideoxy sequencing, the gel pattern shown in this diagram is obtained.What was the sequence of the DNA strand that acted as the template in the sequencing reaction?

• A)5' GCTAGCA 3'
• B)5' ACGATCG 3'
• C)5' TGCTAGC 3'
• D)5' CGATCGT 3'
• E)None of the answers is correct.