You Identify an RFLP in Mice by Digesting Genomic DNA

question 62

Multiple Choice

You identify an RFLP in mice by digesting genomic DNA with HindIII enzyme and radiolabeling a piece of probe DNA.Southern blot analysis shows that the probe detects a 2000 bp fragment in one strain of mice and a 4-kb fragment in another strain of mice.The two mice strains are crossed to produce an F₁ generation.Two F₁ siblings are mated to produce a dozen F₂ progeny.You isolate genomic DNA from several F₁ and F₂ individuals, digest with HindIII, and perform a Southern blot with the same probe. How many bands would be seen in the Southern blot of an F₁ individual?

• A)1
• B)2
• C)3
• D)4
• E)Two of the answers are possible.

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