Figure a Below Shows a Restriction Map of a Rare

question 65

Essay

Figure A below shows a restriction map of a rare prokaryotic gene with its direction of transcription indicated by the arrow.Figure B shows the unique restriction sites contained within a plasmid-cloning vector.The blackened region in Figure A represents the amino acid coding sequence of a protein that can be used in humans as a vaccine.The stippled region in Figure B is a highly active, constitutive (unregulated)prokaryotic promoter region.Letters indicate the cleavage sites for different restriction enzymes.Known DNA sequences are indicated by short thick lines.Explain how you would isolate and then insert the coding region (Figure A)under the control of the indicated promoter in the cloning vector (FigureB)to produce large amounts of the protein in bacterial cells.Assume that the cloning vector carries the gene for tetracycline (an antibiotic)resistance.Letters represent different restriction enzymes.

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Definitions:

Long-run Equilibrium

A state in which all factors of production can be adjusted, allowing for the optimal allocation of resources in a market or economy over time.

Identical Cost Curves

These are cost curves for different firms that have the same shape and attributes, indicating that the firms have the same cost structure at various levels of output.

Industry Produce

The total output of goods and services produced by all the firms operating in a particular industry.

Short-run Supply Curve

A graphical representation showing the quantity of a good that producers are willing to sell at different prices in the short run, where at least one input is fixed.