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The Full-Length (I

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Essay

The full-length (i.e., containing the entire protein coding region)cDNA for a specific eukaryotic gene in humans is 1500 nucleotides long.You screen a pig genomic library with this cDNA and isolate two genomic clones of different lengths.Both clones are sequenced and found to be 1900 and 2100 nucleotides long, from start codon to stop codon.How would you explain the presence of two genomic clones in pigs, and the discrepancies in their length compared to the cDNA probe?


Definitions:

Achiral

Describing a molecule that is superimposable on its mirror image, thus not exhibiting chirality.

Chiral

Describes a molecule that cannot be superimposed on its mirror image, often leading to different reactions and interactions depending on the spatial arrangement of its atoms.

Enantiomer

A molecule from a duo that resembles its counterpart as a mirror reflection but cannot be perfectly overlaid, similar to the relationship between the left and right hands.

Achiral Molecule

A molecule that is superimposable on its mirror image, lacking chirality or a distinct handedness.

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