An in Vitro Transcription System That Contains a Bacterial Gene

question 77

Multiple Choice

An in vitro transcription system that contains a bacterial gene does not initiate transcription. What is one possible problem?

• A) Histones that were on the DNA when it was isolated from E. coli are blocking access to the template.
• B) There is a mutation in the inverted repeat sequence that prevents a hairpin secondary structure from forming.
• C) There is a mutation at -10, where a promoter consensus sequence is located.
• D) Rho factor has not been added.
• E) TATA-binding protein (TBP) has not been added.

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