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Some restriction enzymes cut at specific and unambiguous sequences (e.g., NotI at GCGGCCGC) . As such, the distance between two such sites in a large random DNA sequence is 48, assuming a 50% G+C DNA content. However, the sequence recognized by XhoII is said to be ambiguous (Pu-GATC-Py, where Pu = any purine and Py any pyrimidine) . What, therefore, is the expected distance between two XhoII sites in a large random sequence?
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Research evidence constitutes findings obtained through systematic investigation and analysis, aimed at supporting or refuting hypotheses within academic or practical contexts, essential for informed decision-making.
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The process of identifying specific, measurable, achievable, relevant, and time-bound objectives to be accomplished.
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Management by Objectives, a strategic management model that aims to improve organizational performance by clearly defining objectives that are agreed upon by both management and employees.
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