Your Student Is Trying to Create a "Gene X" Knockout

question 16

Essay

Your student is trying to create a "Gene X" knockout mouse by introducing a recombinant construct into embryonic stem (ES)cells, selecting transformants, and introducing the transformed cells into mouse blastocysts. He designed his construct as follows:
Neo^R----region of homology to Gene X---HSV tk--- region of homology to Gene X
where Neo^R is the neomycin-resistance gene and HSV tk is the Herpes Simplex virus thymidine kinase gene. He transforms the ES cells and exposes them to G418 and ganciclovir. Explain to your student he failed to recover transformed ES cells.

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Definitions:

Internal Control

A process implemented by a company’s management and board of directors to provide reasonable assurance regarding the achievement of objectives in effectiveness and efficiency of operations, reliable financial reporting, and compliance with laws and regulations.

Pre-numbered

Pre-numbered refers to documents or forms that are sequentially numbered in advance, aimed at preventing fraud and ensuring completeness in record-keeping.

Internal Control

Mechanisms and steps a firm takes to confirm the trustworthiness of its financial and accounting reports, enhance accountability, and stop fraudulence.

Controls

Procedures or policies implemented by a company to safeguard its assets, enhance the reliability of its accounting records, increase efficiency, and ensure compliance with laws and regulations.