You Have Evidence That a Gene, VTE4, of Known Sequence

question 18

Multiple Choice

You have evidence that a gene, VTE4, of known sequence and well-understood transcriptional regulation, is available from another lab. The product of this gene enables seeds to produce a significantly increased amount of vitamin E from a substrate already produced in Brassica napus, the plant that gives us canola oil. To produce canola oil with increased vitamin E, how would you proceed?

• A)Select Brassica plants with the highest titer of the vitamin, isolate the genes responsible, and cause these genes to duplicate.
• B)Use a yeast system to engineer yeasts that produce the vitamin, and get manufacturers to add this to the canola oil during bottling.
• C)Introduce the gene and necessary cis elements into Brassica, along with a reporter gene, then measure the vitamin E produced.
• D)Clone VTE4 in A. tumefaciens and use these bacteria to produce transgenic Brassica.
• E)Introduce VTE4 into tomato plants or another food that humans eat more than Brassica.

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