For the Following Gene, MspI and HpaII Restriction Sites and Their

question 45

Multiple Choice

For the following gene, MspI and HpaII restriction sites and their positions are indicated here. Recall that HpaII is methylation sensitive, while MspI is not. If this gene is methylated, which of the following combinations of DNA fragments would you expect from this restriction digest?

• A)5 bands = 100 bp, 200 bp, 1000 bp, 1500 bp, and 1800 bp.
• B)4 bands = 100 bp, 200 bp, 800 bp, and 900 bp.
• C)3 bands = 200 bp, 500 bp, and 1300 bp.
• D)1 band = 2000 bp.
• E)No bands = DNA will be completely digested.

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