You Have Isolated DNA from Actively Replicating Cells

question 25

Multiple Choice

You have isolated DNA from actively replicating cells. Where would you expect to find this DNA on a gel?

• A)DNA would be highly supercoiled and would be found in the band seen in the positive pole (bottom)of Lane 1.
• B)DNA would be predominantly relaxed and would be found in the band seen in the positive pole (bottom)of Lane 1.
• C)DNA would be highly supercoiled and would be found in the band seen in the negative pole (top)of Lane 1.
• D)DNA would be predominantly relaxed and would be found in the band seen in the negative pole (top)of Lane 1.
• E)DNA would be both relaxed and supercoiled, so it would appear in a pattern similar to Lane 2.

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Definitions:

Provoke

To stimulate or incite someone to action or feeling, often with the intent of eliciting a specific reaction.

Proactively

describes taking initiative by acting in advance of a future situation, rather than just reacting, to achieve better results.

Impending Impasse

An approaching stalemate or deadlock situation where progress seems difficult or impossible.

Confrontation

A direct engagement or clash between parties over divergent interests, perspectives, or opinions.